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Accueil du site > Production scientifique > Photodissociation and Dissociative Photoionization Mass Spectrometry of Proteins and Noncovalent Protein–Ligand Complexes

Photodissociation and Dissociative Photoionization Mass Spectrometry of Proteins and Noncovalent Protein–Ligand Complexes

Date de publication: 5 août 2013

F. Canon, A. R. Milosavljevic, G. van der Rest, M. Réfrégiers, L. Nahon, P. Sarni-Manchado, V. Cheynier, A. Giuliani
Angew. Chem. Int. Ed. 52 8377–8381 (2013). DOI

Travail réalisé sur le site de l’Ecole Polytechnique.

Abstract

Keywords : dissociative photoionization ;mass spectrometry ;proteins;tannins ;VUV photon activation

Weight of evidence : Using synchrotron radiation, photo-fragmentation of an intrinsically disordered protein is probed and compared with classical tandem mass-spectrometry activation techniques. It provides excellent sequence coverage allowing the identification of the protein noncovalent binding sites.